Fig 4. Amino acid substitutions identified in the PBPs (1A, 2B and 2X) and MurE of the ST199 isolates in this study (bold letters) and of other isolates published elsewhere (italic letters).

‘Others’ indicates the protein sequence of all merged isolates of this study, except for those indicated by their ID. The nonsusceptibility bases on the epidemiological cut-off (ECOFF) > 0.06 mg/L that indicates the breakpoint between the susceptible (s) and nonsusceptible isolates (ns). The position of the conserved motif SXXK of the PBPs is indicated by asterisks. The amino acid positions, as well as the TP-domain of the respective PBPs shown as bars, are indicated above the sequences. Sequences published elsewhere (italic letters) refer to [35, 37, 41–44].