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The stomach of the rat is divided into the forestomach (pars proventricularis) and glandular stomach (pars glandularis). For this experiment, the proximal glandular stomach of F344 rat was used after removing forestomach. Stomach was opened along the greater curvature and about a one-centimeter length of proximal glandular stomach (corpus) was used for immunohistochemistry (rectangular box in the right side).
