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. 2017 Jan 3;12(1):e0169073. doi: 10.1371/journal.pone.0169073

Fig 2. Gut lipase activity declines with aging in boss mutant flies.

Fig 2

(A) Oil-Red-O staining of neutral lipids in the guts of young (10-days) and old (30-days) control and boss mutant female flies. Anterior midgut region stores lipid (arrowhead). (B) Expression of gut lipases (lipA/margo, CG6295, and dlip4) in the intestines of young (day 10) flies, as assessed by qRT-PCR (n = 3, 5 guts per replicate). (C) boss mutant flies are resistant to orlistat treatment. Young adult male boss mutant flies and control flies were fed a diet with or without orlistat (2.0 μM) for 5 days. Afterward TAG levels were determined (n = 5, 10 flies per replicate) and normalized for total protein. Levels are presented as normalized to levels for wild-type flies. Data are mean relative ratios ± SEM, and differences are significant by t-test (*P<0.05).