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. 2017 Jan 3;12(1):e0169073. doi: 10.1371/journal.pone.0169073

Fig 3. Oxidative stress is enhanced in boss mutant flies.

Fig 3

(A) ALEs were detected by Western blot and antibodies against 4-hydroxy-2-nonenal (4HNE). Ages (0~50-days old) are shown at the top of the blot. (B) Accumulation of fluorescent AGEs was measured in control and boss mutant flies (n = 3, 20 flies per replicate). Data are mean relative fluorescence ± SEM, and differences are significant by t-test (*P<0.05, **P<0.01). (C) Expression of ROS-inducible genes (dMRP4 and gstD1) in young (7-days) and old (35- days) flies, assessed by qRT-PCR (n = 3, 10 flies per replicate). Data are mean relative expression ± SEM, and differences are significant by t-test (*P<0.05).