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. 2017 Jan 3;12(1):e0169073. doi: 10.1371/journal.pone.0169073

Fig 5. SOD2 overexpression rescues aging phenotype of boss mutant flies.

Fig 5

(A) Survival curves of SOD2-overexpressing boss mutant flies (Act-Gal4, UAS-RFP/ UAS-SOD2; boss-/-) and boss mutant flies (Act-Gal4, UAS-RFP/ +; boss-/-) treated with 10 mM paraquat. SOD2 overexpression prolonged the survival of boss mutant flies, shifting the survival curve to the right of the population of SOD2-overexpressing boss mutant flies (n = 30 for each genotypes). Error bars represent S.E. (B) ALEs were detected by Western blot and anti-4HNE antibody. SOD2 overexpression reduced the staining intensity for ALEs (n = 3, 10 flies per replicate). (C) Oil-Red-O staining for neutral lipids in the guts of young (10-days) SOD2-overexpressing control and boss mutant female flies. Anterior midgut region stores lipids (arrowhead). (D) Lifespan for male SOD2-overexpressing boss mutant and boss mutant flies. SOD2 overexpression prolonged the survival of boss mutant flies. Survival was presented by Kaplan Meier curves of SOD2-overexpressing boss mutant and boss mutant flies. n = 50 for each genotypes. Median survival for SOD2-overexpressing boss mutant = 51 days, boss mutant males = 48 days (Log-rank test, p = 0.044).