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. 2017 Jan 3;12(1):e0168888. doi: 10.1371/journal.pone.0168888

Fig 5. Cell proliferation and cell cycle analysis of THP-1 cells exposed to CCL2.

Fig 5

(A) THP-1 cells were seeded on a 96-well plate and incubated with various concentrations of recombinant human CCL2 (10, 50 and 100 ng/ml) for 24 hours and cell proliferation measured by FCM. (B) THP-1 cells were cultured with or without CCL2 (50 ng/ml) for 24 hours. Leukemia cells were harvested, fixed and stained with PI and analyzed by FCM. Each bar represents the mean ± SD of 3 independent experiments (*p < 0.05; **p < 0.01).