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. 2017 Jan 3;12(1):e0169410. doi: 10.1371/journal.pone.0169410

Fig 3. Time course activation of S. scapterisci IJs.

Fig 3

IJs were exposed to 50% house cricket homogenate for different amounts of time and were then quantified for activation. Three biological replicates of triplicate experiments were performed for each time point (n≥249 nematodes per replicate; n≥891 nematodes total per time point) (S1 File). S. scapterisci IJs activate in a time-dependent manner when exposed to house cricket homogenate (p<0.0001; one-way ANOVA). 12h, 18h, 24h, and 30h exposure led to significantly more activation than 6h exposure (p<0.0001; unpaired t test). 18h, 24h, and 30h exposure led to significantly more activation than 12h (p≤0.0002; unpaired t test). However, there was no significant difference in IJ activation between 18h, 24h, and 30h exposure times (unpaired t test). There we no significant differences in the ratio of partially activated IJs in the time course experiments (one-way ANOVA).