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. 2015 Oct 20;2(4):439–446. doi: 10.3233/JND-150111

Fig.2.

Fig.2

Specificity of the α-alanine antibody. A) Immunoblot of lysates from HEK 293 cells transfected with empty plasmid (Ctrl) or plasmid encoding His-tagged wild type (A10) PABPN1 or PABPN1 expanded to 13–17 alanines probed with the α-Alanine antibody. Blots were probed with α-His to confirm expression of recombinant protein and α-PABPN1 to detect PABPN1 protein. An α-Tubulin antibody was used to detect tubulin as a loading control. B) Immunoblot of lysates from untransfected HEK 293 cells (Ctrl) or HEK 293 cells transfected with pTL-1 plasmid encoding HA-tagged wild type (A17) RUNX2 or alanine-expanded (A27) RUNX2 probed with the α-Alanine antibody. Blots were probed with α-HA to confirm protein expression and α-HSP90 was used to detect HSP90 as a loading control.