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. 2015 Oct 20;2(4):439–446. doi: 10.3233/JND-150111

Fig.4.

Fig.4

Use of the α-alanine antibody in a transgenic mouse model of OPMD. A) Immunoblots of whole muscle lysate from mice expressing transgenic wild type (A10.1) or alanine-expanded PABPN1 (A17.1) were probed with α-Alanine antibody. Samples were also probed with α-PABPN1 and α-HSP90 as controls. B) Immunostaining of tibialis anterior muscle sections from A10.1 and A17.1 animals stained with α-Alanine antibody. Immunostaining with α-PABPN1 was used to confirm the presence of PABPN1 protein in A10.1 animals and DAPI was used to stain nuclei. Merge image (bottom panels) is included to show co-localization of both A17 and wild type PABPN1 with nuclei. Bar = 5 μm. C) Immunoblots of whole fly lysates from flies expressing muscle-specific A10- or A17-PABPN1 probed with α-Alanine antibody. Samples were also probed with α-PABPN1 to detect PABPN1 and with α-Lamin D to detect lamin D as a loading control.