Table II.
Relative carboxyl methyltransferase activities with various substrates (each at 1 mm concentration) of crude petal extracts of S. floribunda and N. suaveolens, and of the purified enzymes from the two species
S. floribunda Petal Extract | S. floribunda Purified SAMT | N. suaveolens Petal Extract | N. suaveolens Purified BSMT | |
---|---|---|---|---|
SA | 100 (235)a | 100 (13.1)b | 12.8 | 22.5 |
BA | 12.5 | 31.9 | 49.6 | 100 (2.3)b |
3-Hydroxy BA | 7.2 | 75.6 | 18.8 | 15.3 |
4-Hydroxy BA | 2.8 | 34 | 99.3 | 76.2 |
(E/Z) Jasmonic acid | 2.6 | 4.8 | 6.0 | ndc |
(E) Cinnamic acid (CA) | 2.4 | 34.5 | 2.0 | 16.5 |
(E) 2-Hydroxy CA | 2.7 | 25.0 | 6.0 | 21.0 |
(E) 3-Hydroxy CA | 2.6 | 23.8 | 0 | 4.9 |
(E) 4-Hydroxy CA | 2.9 | 4.4 | 1.5 | 2.55 |
Vanillic acid | nd | 17 | nd | nd |
Caffeic acid | nd | 32.5 | nd | nd |
2,3-Hydroxy BA | 68.9 | 99.5 | 13.5 | 14.9 |
2,4-Hydroxy BA | 9.0 | 98.5 | 20.3 | 18.2 |
2,5-Hydroxy BA | 17.9 | 1.5 | 0 | 2.4 |
2,6-Hydroxy BA | 1.1 | 1.0 | 0 | 1.6 |
3,4-Hydroxy BA | 3.4 | 27.9 | 26.3 | 5.7 |
3,5-Hydroxy BA | 3.3 | 2.5 | 2.6 | 1.8 |
2-Methoxy BA (o-anisic acid) | 3.4 | nd | 100 (4.4)a | 83.9 |
Numbers in parentheses represent activities in pkat/g FW.
Numbers in parentheses represent specific activities in pkat/mg protein.
nd, Not determined.