Figure 2.
Expression in E. coli of recombinant LjEβOS protein and GC-MS analysis of monoterpene products formed by recombinant LjEβOS enzyme activity in vitro. Total ion chromatogram and mass spectra are of LjEβOS in vitro enzyme activity products with GPP as substrate. The mass spectrum of the most abundant product (Peak II) and a minor compound (Peak I), identified as (E)- and (Z)-β-ocimene, respectively, are shown together with that of the respective of authentic standards.