Table I.
Kinetic parameters of petunia BPBT
| Substrate | Km | Vmax | Kcat | Kcat/Km |
|---|---|---|---|---|
| μm | nkat/mg | s−1 | mm−1 s−1 | |
| Acetyl-CoA | 682 ± 68 | 15.0 ± 3.1 | 4.4 ± 0.9 | 6.68 ± 1.6 |
| Benzoyl-CoAa | 1555 ± 108 | 194 ± 10 | 57.1 ± 3.1 | 37.2 ± 2.7 |
| Benzoyl-CoAb | 875 ± 134 | 279 ± 48 | 81.3 ± 14 | 92.2 ± 2.5 |
| Benzyl alcoholc | 28.6 ± 3.5 | 5.52 ± 0.7 | 1.61 ± 0.2 | 56.8 ± 5.5 |
| Benzyl alcohold | 444 ± 44 | 125.5 ± 6 | 36.9 ± 1.8 | 83.1 ± 3.9 |
| Phenyl ethanole | 686 ± 60 | 122.9 ± 3 | 35.8 ± 1 | 52.8 ± 3.6 |
a
Benzoyl-CoA with benzyl alcohol.
b
Benzoyl-CoA with phenyl ethanol.
c
Benzyl alcohol with acetyl-CoA.
d
Benzyl alcohol with benzoyl-CoA.
e
Phenyl ethanol with benzoyl-CoA.