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. 2017 Jan 3;12(1):e0169562. doi: 10.1371/journal.pone.0169562

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© 2017 Mori et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) Schematic diagram of N-glycosylation sites on Gusb. (B) The importance of glycosylation for binding of Dectin-2. Flag-tagged Gusb were immunoprecipitated with biotinylated anti-Flag (M2) antibody followed by enrichment with streptavidin beads. After incubation of Flag-tagged Gusb proteins with Dectin-2-Ig, co-precipitated Dectin-2-Ig was detected by western blotting using anti-hIg mAb.