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. 2004 Aug;135(4):2012–2024. doi: 10.1104/pp.104.048116

Figure 6.

Figure 6.

GC-MS profiles of products formed by the heterologously expressed sesquiterpene synthase CsαFS and CsβCS with FDP as substrate. Chromatogram of product (m/z 93 + 161 + 189 + 204) obtained from assay with lysate of bacteria expressing recombinant CsαFS. The main product peak (Rt 13.95) is (E,E)-α-farnesene (A). Chromatogram of product (m/z 93 + 161 + 189 + 204) obtained from assay with lysate of bacteria expressing recombinant CsβCS. The main product peak (Rt 12.98) is (E)-β-caryophyllene by comparison with the Wiley GC-MS database (B). Chromatogram (m/z 93 + 161 + 189 + 204) of assay with lysate of bacteria expressing pET23c (empty vector; C). Mass spectrum of peak at 13.95 (D). Mass spectrum of peak at 12.98 (E). Compounds were identified by comparison with the Wiley GC-MS database, Adams (1995) and Joulain and König (1998).