Fig 3. Phylogenetic trees showing the evolutionary relationship of the calreticulin family of proteins in vertebrates, rooted by the mammalian calreticulin-3.

The tree was used to name the calreticulin isoforms of Salmo salar. The tree was constructed using multiple alignments of the vertebrate calreticulin. The neighbour-joining method in MEGA 5 [37] was selected. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (10,000 replicates) is shown next to the branches [40]. The salmon sequences are boxed. Each entry is described by the species common name and protein name, followed by the Genbank or Ensembl accession number.