Table 2. Primer sequences, together with their optimum annealing temperature and product size, used for the gene expression studies in Atlantic salmon skin cDNA.

Gene		Primers	Sequence (5’-3’)	PS*/ bp	Ann.† T/°C	E% §	R2¤
Name	Accession
Elongation factor 1α	BT060384	EEF1a_F	CAAGGATATCCGTCGTGGCA	313	61	102	0.990
		EEF1a_R	ACAGCGAAACGACCAAGAGG
β-actin	BT047241	ACTB_F	ATGGAAGATGAAATCGCCCC	239	61	102	0.986
		ACTB_R	TGCCAGATCTTCTCCATGTCG
Calreticulin-like	BT072764	CALRL_F	CCGCTGACTCTACCATCTACAA	192	62	101	0.993
		CALRL_R	CTTGTCATCTTCCTCCTGTTTC
Calreticulin	BT044674	CALR_F	AACATTGGAGTGTTGGGTCTGG	173	67	102	0.996
		CALR_R	TTTCCTCTCCTCCTCCTCCTGT
60S acidic ribosomal	BT059903	RPLP0_F	GTTGCTGCCTCACATCAAAG	211	59	87	0.990
protein P0		RPLP0_R	AGATCTTGGTGGTGATGCC
Hemopexin-like protein	Z68112	HPXL_F	AGAGGCCACCACTTCCTGGA	225	66
		HPXL_R	TCCACTCCCAGCACCTCCTT
40kDa peptidyl-prolyl	BT046523	PPID_F	GAAGCCATGTGTAATTGCTGAG	240	62	102	0.990
cis-trans isomerase		PPID_R	CAGATACCTGAGAGCTTTGGAGT
Keratin, type I	AJ427868	KRT13_F	GAAGGAGGAGCTCATCTATCTCAAG	209	60	99	0.997
cytoskeletal 13		KRT13_R	GTCTCCGTCTTGGTCTGGAA
Glyceraldehyde-3-	BT045621	GAPDH_F	ATGACCACAGTCCACGCCTA	169	65	81	0.991
phosphate dehydrogenase		GAPDH_R	ATGCCAGTCAGCTTGCCGTT

* PS = product size

^†Ann. = annealing

^§ E% = primer efficiency

^¤ R² = coefficient of determination.