Table 3. Differentially expressed proteins in the skin of Atlantic salmon fed prebiotic dietary supplements, as identified by two-dimensional electrophoresis.
The relevant spots were cut out from the gel and sequenced by LC-MS/MS, after which the peptides were identified by a MASCOT search. The spot numbers refer to Fig 1, MW stands for molecular weight and fold change represents the expression level in the experimental fish group as compared to the control.
| Spot # | Accession number | Protein ID | MW/Da | pI | MASCOT results | Fold change | p-value | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Matched Peptides | Sequence coverage/% | Score | ||||||||
| 680 | GI:1848139 | Hemopexin-like protein (Oncorhynchus mykiss) | 76,688 | 6.25 | 3 | 5 | 173 | +1.8 | 0.048 | |
| 757 | GI:224613524 | Calreticulin precursor (Salmo salar) | 30,030 | 4.51 | 15 | 34 | 691 | +1.3 | 0.080 | |
| 2020 | GI:209736184 | 60S acidic ribosomal protein P0 (Salmo salar) | 31,083 | 9.10 | 35 | 62 | 1002 | +1.3 | 0.017 | |
| 2198 | GI:185133596 | Type I keratin E7 (Oncorhynchus mykiss) | 23,095 | 5.11 | 11 | 19 | 240 | -2.3 | 0.023 | |
| 845 | GI:185135325 | Keratin, type I cytoskeletal 13 (Oncorhynchus mykiss) | 71,984 | 7.72 | 8 | 21 | 277 | -2.1 | <0.001 | |
| 921 | GI:185135325 | Keratin, type I cytoskeletal 13 (Oncorhynchus mykiss) | 71,984 | 7.72 | 21 | 30 | 824 | -2.0 | 0.003 | |
| 2002 | GI:185132221 | Type II keratin E1 (Oncorhynchus mykiss) | 97,540 | 8.68 | 6 | 9 | 350 | -2.0 | 0.015 | |
| 2033 | GI:185135325 | Keratin, type I cytoskeletal 13 (Oncorhynchus mykiss) | 71,984 | 7.72 | 39 | 44 | 1215 | -1.8 | 0.021 | |
| 2007 | GI:185135325 | Keratin, type I cytoskeletal 13 (Oncorhynchus mykiss) | 71,984 | 7.72 | 22 | 23 | 798 | -1.7 | 0.005 | |
| 2072 | GI:185132221 | Type II keratin E1 (Oncorhynchus mykiss) | 97,540 | 8.68 | 36 | 34 | 1224 | -1.7 | <0.001 | |
| 862 | GI:185133596 | Type I keratin E7 (Oncorhynchus mykiss) | 31,541 | 5.04 | 12 | 16 | 330 | -1.5 | 0.043 | |
| 971 | GI:209730456 | 40 kDa peptidyl-prolyl cis-trans isomerase (Salmo salar) | 31,499 | 5.42 | 18 | 50 | 682 | -1.5 | 0.016 | |
| 1043 | GI:185132746 | Glyceraldehyde-3-phosphate dehydrogenase (Oncorhynchus mykiss) | 51,406 | 8.90 | 47 | 56 | 853 | -1.4 | 0.027 | |