Table 4. Validation of proteomic results by analysing the gene expression of the skin mRNA corresponding to the respective proteins.
Fold changes at the proteomic level was calculated on SameSpots using normalised spot volume data of two-dimensional gels (2DGE). Gene expression fold changes were calculated from Ct values of qPCR assays by deriving arbitrary units using the standards graph equation and normalising using two housekeeping genes, EF-1α and β-actin. (n = 9).
| Trial#1 | Trial#1 | Trial#2 | ||
|---|---|---|---|---|
| Primer | 2DGE fold change | P value | qPCR fold change | |
| CALR | +1.2 | 0.40 | n.s | n.s. |
| CALRL | +1.3 | 0.08 | +1.26† | +1.72* |
| GAPDH | -1.4 | 0.03 | n.s. | n.s. |
| HPXL | +1.8 | 0.05 | n.e. | n.e. |
| KRT13 | -1.8 | 0.02 | n.s. | n.s. |
| PPID | -1.5 | 0.02 | n.s. | n.s. |
| RPLP0 | +1.3 | 0.02 | n.s. | +1.39* |
† p<0.075
* p<0.05
n.s. = not significant; n.e. = not expressed.