Skip to main content
. 2016 Dec 15;12(12):e1006518. doi: 10.1371/journal.pgen.1006518

Fig 3. BrafV600E induces ER stress and reduced PERK gene dosage attenuates BrafV600E-dependent senescence in premalignant skin.

Fig 3

A) BrafV600E-dependent regulation of ER stress genes (CHOP, Grp78, Grp94, ATF4, GADD34, ERDJ4, XBP1) in primary melanocytes (QPCR; *p<0.05; p-values analyzed by two-tailed Student t test) B) β-galactosidase staining of primary BrafWT vs BrafV600E melanocytes. (Scale bars 50μm; *p<0.05; p-values analyzed by two-tailed Student t test); western analysis of cell lysates using indicated antibodies. C) UPR gene expression assay (QPCR) for Chop, Xbp1, Bip in premalignant skin from BrafWT or BrafV600E mice. D) β-galactosidase assay in premalignant skin isolated from TyrCreCA/+; BrafV600ECA/+; Perk+/+ or Perk+/- or Perk-/- mice. Arrowheads indicate positive staining. Scale bars = 50μm. E) Detection of cyclin D1 in the tissues indicated by immunoblot. Total Erk1/2 is provided as a loading control. The line denotes excision of irrelevant lanes. F) Quantification of E. Cyclin D1 protein levels expressed as a ratio relative with total ERK.