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. 2016 Dec 15;12(12):e1006518. doi: 10.1371/journal.pgen.1006518

Fig 7. Characterization of a tumor-derived mutation of the PERK kinase.

Fig 7

A) Depection of melanoma-derived PERK mutations. B-D) Western analysis of MEF lysates expressing murine versions of Perk alleles tested under ER-stress conditions. E) Clonogenic survival assay of Perk-/- fibroblasts reconstituted with Perk alleles (indicated on left) and treated with with chemicals indicated at the top. Cells stained with Geimsa after 14 days post-treatmen. F) Quantification of colonies that formed in soft agar for Perk-/- fibroblasts reconsituted with the indicated Perk mutants. G) Tumor-derived Perk alleles exhibit dominant negative activity.