Table 1. Competitive Inhibition ELISA of Serum Antibodies from Diabetes Type 2 Patients.
Sera No | Maximum percent inhibition at 20 μg/ml | |||
---|---|---|---|---|
N-IgG | OH•-IgG | MG-IgG | OH•-MG-IgG | |
9 | 12.4 | 47.1 | 52.2 | 66.4 |
10 | 30.3 | 50.4 | 55.4 | 64.9 |
12 | 25.2 | 42.5 | 47.3 | 67.3 |
19 | 22.2 | 47.1 | 52.2 | 62.6 |
20 | 26.4 | 50.1 | 48.6 | 60.6 |
27 | 22.2 | 55.6 | 51.5 | 68.2 |
35 | 25.9 | 44.4 | 51.6 | 61.9 |
36 | 29.0 | 51.6 | 48.3 | 68.0 |
41 | 21.6 | 51.6 | 49.5 | 66.3 |
44 | 24.4 | 46.4 | 55.2 | 64.9 |
50 | 31.7 | 50.4 | 46.8 | 67.4 |
51 | 21.7 | 49.8 | 48.9 | 59.1 |
59 | 29.2 | 42.2 | 45.8 | 52.5 |
61 | 31.5 | 55.5 | 49.4 | 63.4 |
63 | 32.4 | 56.9 | 50.6 | 69.1 |
65 | 27.4 | 56.6 | 51.5 | 67.1 |
70 | 23.6 | 52.0 | 45.8 | 67.6 |
71 | 34.7 | 56.4 | 50.7 | 65.4 |
Mean±SD | 26.2±5.3 | 50.3±4.7 | 50.1±2.8 | 64.6±4.1 |
The microtitre plates were coated with 10 μg/ml of OH•-MG-IgG