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. 2016 Nov 29;5:e17361. doi: 10.7554/eLife.17361

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© 2016, Jaitner et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 2. — (A) In a contextual fear conditioning paradigm, Satb2 cKO mice (cKO), showed (i) similar levels of freezing to Satb2^flox/flox mice (Ctrl) during the fear-acquisition phase (cKO, n = 7; Ctrl, n = 6; repeated measures ANOVA, F_3,33 = 0.76, p = 0.52) and at the 1 hr fear expression test (Student's t test, t₁₁ = 0.19, p = 0.86) but (ii) froze significantly less than their littermate controls at the 24 hr fear expression test (cKO, n = 8; Ctrl, n = 8; repeated measures ANOVA, F_3,42 = 0.36, p = 0.778; Student's t test, t₁₄ = 4.88, p = 0.0002). Data are presented as mean ± SEM, n values refer to the number of mice per group, *p < 0.05. (B) Object location memory test. (i) Scheme of the experiment. (ii) Satb2 cKO mice (n = 11) and control mice (n = 10) exhibited similar preference for the novel location over the familiar location at the 1 hr memory retention test (Student's t test, t₁₉ = 0.46, p = 0.65). (iii) Satb2 cKO mice (n = 8) showed reduced preference for the novel location over the familiar location at the 24 hr memory retention test (Student's t test, t₁₄ = 2.89, p = 0.011) compared to Satb2^flox/flox mice (n = 8). The relative exploration time is expressed as a percent discrimination index (D.I. = (t_{novel location} − t_{familiar location}) / (t_{novel location} + t_{familiar location}) × 100%). Data are presented as mean ± SEM, n values refer to the number of mice, **p < 0.01. (C) Novel object recognition test. (i) Scheme of the experiment. (ii) Satb2 cKO mice (n = 10) and control mice (n = 9) exhibited a similar preference for the novel over the familiar object at the 1 hr memory retention test (Student's t test, t₁₉ = 1.11, p = 0.28). (iii) Satb2 cKO mice (n = 8) spent less time exploring the novel object at the 24 hr memory retention test (Student's t test, t₁₄ = 3.0, p = 0.009) compared to Satb2^flox/flox mice (n = 8). The relative exploration time is expressed as a percent discrimination index (D.I. = (t_{novel object}− t_{familiar object}) / (t_{novel object}+ t_{familiar object}) × 100%). Data are presented as mean ± SEM, n values refer to the number of mice, *p < 0.05.

DOI: http://dx.doi.org/10.7554/eLife.17361.005

Figure 2—figure supplement 1. — (A) In a contextual fear conditioning paradigm, Satb2 cKO mice (cKO), showed (i) similar levels of freezing to Satb2^flox/flox mice (Ctrl) during the fear-acquisition phase (cKO, n = 7; Ctrl, n = 6; repeated measures ANOVA, F_3,33 = 0.76, p = 0.52) and at the 1 hr fear expression test (Student's t test, t₁₁ = 0.19, p = 0.86) but (ii) froze significantly less than their littermate controls at the 24 hr fear expression test (cKO, n = 8; Ctrl, n = 8; repeated measures ANOVA, F_3,42 = 0.36, p = 0.778; Student's t test, t₁₄ = 4.88, p = 0.0002). Data are presented as mean ± SEM, n values refer to the number of mice per group, *p < 0.05. (B) Object location memory test. (i) Scheme of the experiment. (ii) Satb2 cKO mice (n = 11) and control mice (n = 10) exhibited similar preference for the novel location over the familiar location at the 1 hr memory retention test (Student's t test, t₁₉ = 0.46, p = 0.65). (iii) Satb2 cKO mice (n = 8) showed reduced preference for the novel location over the familiar location at the 24 hr memory retention test (Student's t test, t₁₄ = 2.89, p = 0.011) compared to Satb2^flox/flox mice (n = 8). The relative exploration time is expressed as a percent discrimination index (D.I. = (t_{novel location} − t_{familiar location}) / (t_{novel location} + t_{familiar location}) × 100%). Data are presented as mean ± SEM, n values refer to the number of mice, **p < 0.01. (C) Novel object recognition test. (i) Scheme of the experiment. (ii) Satb2 cKO mice (n = 10) and control mice (n = 9) exhibited a similar preference for the novel over the familiar object at the 1 hr memory retention test (Student's t test, t₁₉ = 1.11, p = 0.28). (iii) Satb2 cKO mice (n = 8) spent less time exploring the novel object at the 24 hr memory retention test (Student's t test, t₁₄ = 3.0, p = 0.009) compared to Satb2^flox/flox mice (n = 8). The relative exploration time is expressed as a percent discrimination index (D.I. = (t_{novel object}− t_{familiar object}) / (t_{novel object}+ t_{familiar object}) × 100%). Data are presented as mean ± SEM, n values refer to the number of mice, *p < 0.05.

DOI: http://dx.doi.org/10.7554/eLife.17361.005