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In vitro phosphorylation of starch granules by recombinant GWD. Starch was extracted from leaves harvested at the times indicated. Granules (2 × 5 mg each) were incubated with 25 μm ATP containing 1.2 106 cpm [βγ-33P]ATP and 1 μg of GWD for 50 min. Gray and black bars represent the individual measurements of duplicate determinations. The radioactivity present in control samples lacking GWD was subtracted.
