Figure 2.

Two-dimensional protein patterns of leaves obtained from a constant fresh-tissue load. An equal volume of 80 μL corresponding to 240 mg of fresh weight of leaves harvested at 4, 12, and 24 DAP of the second flowering node was loaded on linear 24-cm IPG strips (pH 4–7). Gels were Coomassie Blue stained. The figure shows representative experiments carried out at three times. A, Enlarged image of massive degradation of rbcL between 4 and 24 DAP. B, Enlarged image represents some less abundant protein spots (labeled by arrows) with constant levels between 4 and 24 DAP.