Figure 1.

Genomic organization of the pgip gene family in bean genotype BAT93. A, Southern-blot analysis of genomic DNA from line BAT93 and plasmid DNA from 129F4 and 10G1 BAC clones; DNA was double digested with EcoRI-HindIII and probed with a fragment corresponding to the portion of the PvPpgip1 ORF encoding the mature protein. The 5.5-kb hybridizing fragment is present in both BAC clones. B, Schematic representation of the arrangement of the PvBpgip genes as determined by sequencing the 129F4 and 10G1 BAC clones. The two clones overlap for about 5.5 kb; this region includes PvBpgip2. Numbers between pgip members represent distances in kb. Arrows indicate the direction of the coding region from ATG to stop codon. H, HindIII restriction site. Numbers in brackets indicate the position of the terminal HindIII sites in each BAC clone, where numbering starts from the HindIII site following the T7 promoter sequence of pBeloBAC11. In the overlapping region, the positions of the corresponding HindIII sites in the two BAC clones are also indicated.