Figure 3.

ER degradation limits untagged mOREG functional expression. CNG cells transiently transfected with untagged mOREG were assayed for increases in intracellular calcium in response to increasing concentrations of eugenol (from 3 uM to 1000 uM) following treatment with vehicle (control), 10 mM 3-MA, or 50 uM MG-132 for 4 h. The EC₅₀ values for eugenol for control (36.5 +/- 12.9 uM), 3-MA (42.6 +/- 6.5 uM), and MG-132 (36.4 +/- 7.5 uM) treatments were not significantly different. CNG cells transfected with vector only (pUC18) and treated with vehicle, 3-MA, or MG-132 did not respond to 1000 uM eugenol.