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. 2004 Sep 15;5(1):12. doi: 10.1186/1465-9921-5-12

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Copyright © 2004 Koth et al; licensee BioMed Central Ltd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Aspergillus antigen-treated wild-type, Mcp-1^-/-and Ccr2^-/-mice demonstrated intact Th2 cytokine production and induction of IgE. For cytokine determination, draining lymph node cells from Aspergillus antigen-treated wild-type, Mcp-1^-/-and Ccr2^-/-mice were isolated and stimulated with PMA/ionomycin for 40 hr and cytokine levels for IL-4 (A), IL-5 (B), IL-13 (C), and IFN-γ (D) were quantitated by ELISA. Serum IgE (E) from Aspergillus antigen-treated wild-type, Mcp-1^-/-and Ccr2^-/-mice and control mice were measured by ELISA. In (A-D), bars represent mean ± SE; in (E), results are expressed as the common log of IgE concentration where each circle represents a single PBS- or Aspergillus antigen-treated mouse and horizontal lines represent the mean of each group (PBS-treated, N = 5 mice/group; Aspergillus antigen-treated, N = 8–9 mice/group). Aspergillus antigen exposure and sample collection are described in methods. Asterisks (*) indicate values that are statistically significantly different (p < 0.001) compared to PBS controls.