TABLE 2.
Primer sequences
| Primer | Nucleotide sequence (5′-3′) | Target | Reference(s) |
|---|---|---|---|
| amoA-1F | GGG GTT TCT ACT GGT GGT | amoA gene of betaproteobacteria AOB | 44 |
| amoA-2R-TC | CCC CTC TGC AAA GCC TTC TTC | amoA gene of betaproteobacteria AOB | 31, 44 |
| amoA-1F-clamp | (CGCCGCGCGGCGGGCGGGGCGGGGGCACGGGGG)-GGG GTT TCT ACT GGT GGTa | amoA gene of betaproteobacteria AOB | 31 |
| Eub 338 F | ACT CCT ACG GGA GGC AGC | Bacteria | 1 |
| Univ 907 R | CCG TCA ATT CCT TTR AGT TT | Bacteria | 30 |
a
The clamp portion of the sequence is shown in parentheses.