TABLE 3.

Complementation analysis of the noninfectious OB phenotype^a

Expt	DNA used to infect cells	Plasmid	Genes complemented	Total no. of larvae	No. of dead larvae	Rescue
1	SfNIC-B			47	47	+
2	SfNIC-C or -D			37	3	−
3	SfNIC-G			35	3	−
4	SfNIC-B	p264.306	pif, pif-2, and ORF homologue to Se37	44	44	+
5	SfNIC-C or -D	p264.306	pif, pif-2, and ORF homologue to Se37	39	31	+
6	SfNIC-G	p264.306	pif, pif-2, and ORF homologue to Se37	35	1	−
7	None (mock infected)			37	2	−

^aSf9 cells were transfected with viral DNA alone (experiments 1, 2, and 3) or cotransfected with viral DNA and the p264.306 plasmid, which includes the pif and pif-2 genes (experiments 4, 5, and 6). Neonatal larvae were fed OBs produced by the infected cells. At least two independent replicates were performed for each experiment. Dead larvae were examined for the presence of OBs.