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. 2004 Sep;70(9):5546–5556. doi: 10.1128/AEM.70.9.5546-5556.2004

FIG. 4.

FIG. 4.

(A) Northern blot analysis of total RNAs isolated from MG1614 and MG1614/pJO-J at three different temperatures and hybridized to an llaJIM1-specific probe. Lanes 1 and 2, RNAs isolated from MG1614 and MG1614/pJO-J, respectively, at 25°C; lanes 3 and 4, RNAs isolated from MG1614 and MG1614/pJO-J, respectively, at 30°C; lanes 5 and 6, RNAs isolated from MG1614 and MG1614/pJO-J, respectively, at 37°C. The positions of the 16S and 23S rRNAs as well as the RNA markers are indicated. (B) Primer extension analyses of total RNAs isolated from MG1614/pNP40 (lane 1), MG1614 (lane 2), and MG1614/pJO-J (lane 3) at 30°C identified the transcriptional start site as a guanine residue (see Fig. 2B).