TABLE 5.
In vivo LlaJI methylation assaya
| Propagating host | Value for indicated strain
|
|||||
|---|---|---|---|---|---|---|
| MG1614
|
MG1614/pJO-J
|
MG1614/pJO-M1M2R1
|
||||
| EOP | Plaque size (mm) | EOP | Plaque size (mm) | EOP | Plaque size (mm) | |
| NZ9000/pNZ8020 | 1 | 2-3 | 1 × 10−7 | 1-2 | 1 | 1-2 |
| NZ9000/pNZ-M1 | 1 | 2-3 | 3 × 10−1 | 1-2 | 1 | 1-3 |
| NZ9000/pNZ-M2 | 1 | 2 | 4 × 10−1 | 1-2 | 1 | 2 |
| NZ9000/pNZ-M1M2 | 1 | 2-3 | 1 | 2-3 | 1 | 2-3 |
a
φsk1 was propagated on strains overexpressing LlaJI methylases separately and in tandem and was subsequently analyzed for its insensitivity to restriction by pJO-J and pJO-M1M2R1 at 19°C.