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. 2004 Sep;70(9):5244–5251. doi: 10.1128/AEM.70.9.5244-5251.2004

FIG. 5.

FIG. 5.

(A) ldh mRNA level in S. bovis 12U1 grown on glucose (lane a) or lactose (lane b) and ldh mRNA level in S. bovis 12U1-ccpA− grown on glucose (lane c) or lactose (lane d), as estimated by Northern blotting. The arrow indicates the position of 1.0-kb ldh mRNA. (B) pfl mRNA level in 12U1 grown on glucose (lane a) or lactose (lane b) and pfl mRNA level in 12U1-ccpA− grown on glucose (lane c) or lactose (lane d). The arrow indicates the position of 2.3-kb pfl mRNA. (C) Alignment of the ldh (accession number U60997) and pfl (AB014686) promoter regions of S. bovis. The −35 and −10 sequences are underlined. Possible cre sites are enclosed in boxes, and the consensus cre sequence is indicated.