TABLE 1.
Effect of disruption of ccpA on the ratio of formate to lactate produced in S. bovisa
| Strain | Energy substrate | Growth rateb | Relative amt of mRNAc
|
Sp act
|
Ratio of formate to lactatee | |||
|---|---|---|---|---|---|---|---|---|
| ldh | pfl | LDHd | PFLd | PFL/LDH | ||||
| 12U1 | Glucose | 0.98 A | 1.0 A | 1.0 C | 61 A | 1.8 C | 0.03 | 0.07 D |
| Lactose | 0.78 B | 0.5 B | 3.6 B | 29 B | 5.5 B | 0.19 | 0.48 B | |
| 12U1-ccpA− | Glucose | 0.94 A | 0.5 B | 9.4 A | 27 B | 14.2 A | 0.53 | 0.39 C |
| Lactose | 0.82 B | 0.5 B | 9.2 A | 31 B | 14.5 A | 0.47 | 0.59 A | |
| 12U1-ccpA+ | Glucose | 0.97 A | 1.1 A | 1.2 C | 59 A | 1.9 C | 0.03 | 0.09 D |
| Lactose | 0.80 B | 0.5 B | 3.4 B | 31 B | 5.1 B | 0.20 | 0.49 B | |
a
Different letters within a column indicate a significant difference (P < 0.05; n = 3).
b
The growth rate is expressed as an increase in the amount of cell-N (in milligrams) per hour during the log stage.
c
Relative amount of mRNA as estimated by Northern blotting.
d
Increase or decrease in the amount of NADH (in micromoles per minute per 10 μg of cell-N).
e
Ratio of the amount of formate to the amount of lactate produced in 1 h.