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. 2004 Sep;70(9):5119–5131. doi: 10.1128/AEM.70.9.5119-5131.2004

FIG.3.

FIG.3.

Phylogenetic relationships based on 16S rDNA sequences for biocontrol fluorescent pseudomonads and reference bacteria belonging to the genera Pseudomonas and Erwinia. The distance tree was generated by the NJ method with the JC formula by using X. campestris pv. campestris ATCC 33913 (accession number AE012540) as the outgroup. Nodal support was assessed by using 1,000 bootstrap replicates. Only bootstrap values greater than 50% are shown. Scale bar = 0.02 substitution per site. The P. chlororaphis, P. fluorescens, P. syringae, and P. aeruginosa complexes were those defined in Anzai et al. (5). Strains for which the hrcN sequence is available are indicated by a solid diamond for new sequences or an open diamond for hrcN sequences published previously (National Center for Biotechnology Information). The two strains positive by hrcN hybridization but hrcN negative as determined by PCR are indicated by solid circles. Sequences were obtained in this work for Pseudomonas strains KD (accession number AY456697), VS01 (AY456698), VS02 (AY456699), BE07 (AY456700), BE08 (AY456701), K92.46 (AY456702), K94.08 (AY456703), K94.14 (AY456704), K94.23 (AY456705), P3 (AY456706), P97.26 (AY456707), SBW25 (AY456712), and PILH1 (AY456708) and for E. amylovora 22716 (AY456709), 22770 (AY456710), and 23482 (AY456711). The other sequences used were the sequences for pseudomonad strains CHA0 (AJ278812), Q2-87 (AJ278813), F113 (AJ278814), CM1′A2 (AJ417068), Q37-87 (AJ417069), PGNR1 (AJ417071), Pf-5 (AJ417072), PF (AJ417073), Q65c-80 (AJ417074), DR54 (AY622219), and Pf1 (AY622220), P. chlororaphis ATCC 13985 (AF094722) and IFO3904 (D86004), P. aureofaciens (Z76656), P. corrugata 2172 (= ATCC 29736) (D84012), P. brassicacearum (AF100322), P. marginalis (AB021401), P. fluorescens IAM12022 (D84013), P. gessardii (AF074384), P. rhodesiae (AF064459), P. veronii (AB056120), P. caricapapayae ATCC 33615 (D84010), P. syringae pv. phaseolicola (AB001448) and ATCC 19310 (D84026), P. syringae pv. tomato DC3000 (AE016875), P. syringae pv. syringae FG11 (AY242068), P. putida KT2440 (AE016775), ATCC 17642 (AF094744), and IAM1236 (D84020), P. flavescens B62 (V01916), P. straminea (D084023), P. mendocina (D84016), P. pseudoalcaligenes (Z76675), P. oleovorans (D84018), P. alcaligenes (Z76653), P. resinovorans (Z76668), P. aeruginosa PAO1 (AE004844) and ATCC 15442 (AF094718), P. thermaerum (AB088116), Erwinia herbicola (U80202), and E. amylovora BC201 (AF141892) and BC224 (AF140339).