TABLE 2.
Primers used in this work
| Primer | Sequence (5′-3′) | Use | Reference |
|---|---|---|---|
| hrcV and neighboring genes | |||
| HRCV-L | CCGGAATTCTGCG | Construction of hrcV probe | 58 |
| HRCV-R | ATTGTCATGTCGAT | Construction of hrcV probe | 58 |
| T3f2 | GGTTTAGCAGGTCGATAATC | Synthesis of KD-j | This study |
| prottss-1r | GTCGAGCTGACGAAGGAGAG | Synthesis of KD-j | This study |
| T3f5 | CTCGATCACTTATCCGGCTC | Synthesis of KD-l | This study |
| T3r8 | GGCCTTCATGATGACTTCCA | Synthesis of KD-l | This study |
| pKD2cV-1r | GCAATCGCCTAGTGGTGAAC | Synthesis of KD-vq and KD-vn | This study |
| hrpQ-1rR | CCGKTCAGTACGCGTAATTCA | Synthesis of KD-vq | This study |
| hrcN-1r | CTGGGCWGCGTGCTGGAYGG | Synthesis of KD-n | This study |
| hrcN-3rR | GGCAGCAGGGTGTAMACCGA | Synthesis of KD-vn | This study |
| hrcN-4r | CGAGCAGGAYTCGATGAACG | Partial hrcN sequencing | This study |
| hrcN-5rR | CCGGWYTGGTATTCACCCAG | Synthesis of KD-n, partial hrcN sequencing | This study |
| 16S rDNA gene | |||
| PH-16S | AAGGAGGTGATCCAGCCGCA | Sequencing of 16S rDNA gene | 12 |
| PA-16S | AGAGTTTGATCCTGGCTCAG | Sequencing of 16S rDNA gene | 12 |
| Intern 2a | GATGATCAGCCACAC | Sequencing of 16S rDNA gene | This study |