TABLE 2.
Primers used for PCR amplification
| ORF | Internal primersa | External primersb |
|---|---|---|
| La57 | Forward GATCTCTAGA-CCAGCAATCCAGTT | Forward GCATGCCAGCGATAAAGAAT |
| Reverse GATCAGATCT-CTACACCGCTGATG | Reverse AAGGTGGTTCGCTCAGAAAT | |
| La867 | Forward GATCTCTAGA-ATATTGCGGTTGG | Forward GCACCTCAACAAAGTGATCAG |
| Reverse GATCAGATCT-AGTGGGAAACATCG | Reverse AAAGGCCCTTAGATGGAAC | |
| La996 | Forward GATCTCTAGA-AGCCTGAGCCATAC | Forward ACAGTGTAGCCCTTGTAG |
| Reverse GATCAGATCT-AGCGATACCGTTCC | Reverse CCATACTTGGAGGAGAAC | |
| La995 | Forward GATCTCTAGA-TGCTGCGCCTTACA | Forward AGTTGGCCAGTCATTGCT |
| Reverse GATCAGATCT-TAGGGCACCCGTTC | Reverse TCGCCAAGATCACGAAGA |
a
Primer sets used to amplify the internal region for gene inactivation.
b
Primer sets used to amplify junction fragments.