FIG. 2.

Growth of X. nematophila leuB opp mutant strains on peptides. Peptides and leucine (Leu), where indicated, were spread on defined medium plates at a final concentration of 70 μM. The plates were inoculated by scoring with a toothpick coated with the appropriate strain. Three independent colonies each of HGB081 (Leu⁺), HGB731 (leuB), HGB732 (leuB oppA₁), HGB733 (leuB oppA₂), and HGB734 (leuB oppB) were tested; a representative result is shown for each condition. Growth at 96 h after inoculation is shown.