FIG. 1.

(A) Gene map of the 2.456-kb KpnI fragment isolated in this study, which contained the gacA response regulatory gene. The ORFs spanning this region are shown, as is the position of the Tn5 insertion which was isolated in this study in the cloned gene and transferred by homologous recombination into the genome of P. putida WCS 358, creating IBE1 (see text for details). Tn5 was at position 1507 of this fragment, and gacA spanned from position 1177 to position 1707. The ORFs adjacent to gacA displayed high identity to pp4101 and pp4100 of P. putida KT2440, which encoded an acetyl transferase belonging to the GNAT family and a transcriptional regulator belonging to the Cro/Ci family, respectively. (B) Gene map of the 2.683-kb SalI-KpnI fragment isolated in this study, which contained the AHL-dependent system of P. putida WCS358. The locations within this fragment of the ppuI gene (spanning positions 1383 to 790), the rsaL gene (spanning positions 1523 to 1753), and the ppuR gene (spanning positions 2473 to 1757) are shown. Also shown are the positions of the Tn5 insertions in the different Tn5 genomic mutants of P. putida WCS358 isolated in this study. The positions of the genomic Tn5 insertions into the rsaL gene are labeled 3C (Tn5 inserted at position 1640) and 2B (Tn5 inserted at position 1672). The positions of the genomic Tn5 insertions into the ppuR gene are labeled 49 (Tn5 inserted at position 1793), 15 (Tn5 inserted at position 2073), 133 (Tn5 inserted at position 2133), and 50 (Tn5 inserted at position 2292). The work described here was performed by using Tn5 mutants 2B (IBE3) and 50 (IBE2); the sequences of these loci have been deposited in data banks under accession numbers AJ629219 and AJ629218, respectively.