FIG. 2.

(A) Northern blot analysis of transformants overexpressing the bipA gene. Hybridization was performed with a 1,900-bp BamHI probe of the bipA gene. Lane 1, control strain lpr66 (untransformed); lane 2, control strain TGDTh-4, containing the tha expression cassette; lanes 3 to 7, A. awamori transformants overexpressing the bipA gene. (B) Control Northern blot analysis of the same RNA samples performed with a 0.83-kb NcoI-KpnI fragment of the constitutive A. nidulans β-actin gene as the probe.