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. 2004 Sep;70(9):5145–5152. doi: 10.1128/AEM.70.9.5145-5152.2004

FIG. 3.

FIG. 3.

Secreted thaumatin in control strains and A. awamori transformants overexpressing the bipA gene. (A) Immunoblots with anti-thaumatin antibodies (1:15,000). Lane 1, control strain TGDTh-4, containing the tha expression cassette; lane 2, control strain lpr66 (untransformed); lanes 3 to 7, A. awamori TB transformants containing the bipA overexpression cassette; lane 8, purified 22-kDa thaumatin from T. daniellii (50 ng). The molecular masses of size markers are indicated on the left. (B) Levels of extracellular thaumatin measured by ELISA in the A. awamori control strain TGDTh-4 and in transformants overexpressing the bipA gene. The error bars indicate standard deviations for three measurements. (C) Correlation between thaumatin secretion and bipA expression levels in the control strain and different A. awamori transformants overexpressing the bipA gene. The data are expressed in units relative to the control strain levels. From left to right the data points correspond to the following strains: TGDTh-4, TB14, TB13, TB23, and TB65. Note that the thaumatin secretion levels depend strongly on the bipA expression levels.