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. 2004 Sep;70(9):5145–5152. doi: 10.1128/AEM.70.9.5145-5152.2004

FIG. 8.

FIG. 8.

(A) Immunoblots of secreted thaumatin in control strains and A. awamori antisense bipA transformants as revealed by anti-thaumatin antibodies (1:15,000). Lanes 1 and 2, A. awamori control strains lpr66 (untransformed) and TGP-3 (containing a tha expression cassette), respectively; lanes 3 to 8, A. awamori BiPAS transformants containing the bipA antisense cassette; lane 9, purified 22-kDa thaumatin from T. daniellii (50 ng). The molecular masses of markers are indicated on the left. (B) Correlation between thaumatin secretion and antisense bipA expression levels of the control strain and the different A. awamori transformants containing the antisense bipA cassette. The data are expressed in units relative to the control strain levels. From left to right the data points correspond to the following transformants: TGP3, BiPAS7, BiPAS12, BiPAS13, BiPAS8, BiPAS9, and BiPAS16.