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Primers used for amplification of sense and antisense bipA RNA
| Fragment amplified | Oligonucleotide | Sequence | Position in gene |
|---|---|---|---|
| bipA transcript (986 bp) | ORNABiPAS1 | 5′-TTCCGCCATGGTTCTTG-3′ | 608-621 |
| ORNABiPAS2 | 5′-TTGGTCAGGGAACGCTCA-3′ | 1631-1648 | |
| Antisense bipA RNA | ORNABiPAS1 | 5′-TTCCGCCATGGTTCTTG-3′ | 608-621 |
| Opgdha | 5′-TTCCTGTTTTCCTAGATACCCCA-3′ |
The Opgdh oligonucleotide corresponds to the nucleotide sequence between the CTATA and CAAT boxes of the gdhA promoter.
