TABLE 2.
Effect of substrate chain length on activity of cell surface-displayed lipase
| Activity parameter | p-Nitrophenyl caproate | p-Nitrophenyl decanoate | p-Nitrophenyl palmitate |
|---|---|---|---|
| Activity (U)a | 96.9 ± 10.2 | 105 ± 10.6 | 43.3 ± 10.6 |
| Specific activity (U/mg of lyophilized cells) | 1,940 ± 200 | 2,000 ± 200 | 870 ± 200 |
| Relative activity (%)b | 92.3 ± 9.72 | 100 ± 10.2 | 41.2 ± 10.1 |
a
Activity was assayed by adding 0.15 mg of lyophilized cells into the substrate solution consisting of 10 mM substrate in acetonitrile, ethanol, and 50 mM Tris-HCl (pH 8.0) at the volume ratio of 1:4:95.
b
Relative activity was calculated by assuming the activity obtained with p-nitrophenyl decanoate as 100%.