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Levels of GLO expression in wild-type P. cyaneo-fulvum and in recombinant P. pastoris and level of expression of GOD in recombinant P. pastorisa
| Organism | Construct | Promoter | Enzyme | Activity (U/liter) |
|---|---|---|---|---|
| P. cyaneo-fulvum | Wild type | GLO | GLO | 5.0 ± 2.1 |
| P. pastoris | pPICK3.5K(GLO) | AOX1 | GLO | 360 ± 21 |
| P. pastoris | pGIC(GLO) | GAP | GLO | 65 ± 6.4 |
| P. pastoris | pGAPZB(GOD) | GAP | GOD | 12,200 ± 720 |
The data were derived from at least three separate expression experiments.
