TABLE 3.
Experimental set-up and results with the multivariate principal component analysis and the pET system
| Expt no. | IPTG addition (OD600) | Temp (°C) | Duration (h) | Yield of active TrxRa
|
|
|---|---|---|---|---|---|
| mg/liter | mg/liter/OD600 | ||||
| 1 | 2.3 | 37 | 10 | 13.7 ± 6.4 | 3.7 ± 1.6 |
| 2 | 0.4 | 30 | 48 | 1.5 ± 0.5 | 0.5 ± 0.1 |
| 3 | 1.8 | 20 | 40 | 13.6 ± 3.0 | 4.2 ± 1.2 |
| 4 | 1.8 | 30 | 5 | 4.6 ± 3.2 | 1.6 ± 1.3 |
| 5 | 0.7 | 37 | 24 | 3.3 ± 1.5 | 1.3 ± 0.4 |
| 6 | 3.0 | 37 | 48 | 3.0 ± 0.02 | 1.5 ± 0.2 |
| 7 | 1.7 | 20 | 24 | 7.8 ± 0.6 | 3.0 ± 0.2 |
| 8 | 1.8 | 25 | 2 | 2.9 ± 1.3 | 1.1 ± 0.4 |
| 9 | 0.6 | 30 | 16 | 5.3 ± 0.3 | 2.2 ± 0.8 |
| 10 | 0.0 | 37 | 48 | 6.0 ± 5.1 | 1.8 ± 1.2 |
| 11 | 1.8 | 20 | 24 | 13.6 ± 1.6 | 4.6 ± 0.3 |
| 12 | 1.5 | 25 | 16 | 8.6 ± 1.2 | 2.9 ± 0.5 |
a
See Table 2, footnote a.