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Characterization of fractions I and II. (A) Western blot showing detection with antisera raised against HLD1 and EngB, respectively. Anti-HLD1 was made against the hydrophilic domain 1 of CbpA, and anti-EngB was made against EngB. (B) Zymogram analysis with 0.1% carboxymethyl cellulase (CMCase) incorporated into the polyacrylamide. Lane Frc I, fraction I; lane Frc II, fraction II. Molecular weights are indicated on the left in thousands.
