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. 2004 Sep;70(9):5701–5703. doi: 10.1128/AEM.70.9.5701-5703.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 1. — Primers TcPc 173F and TcPc 589R and the corresponding sites of consensus sequences of 16S rRNA genes of organisms related to Thermococcaceae and of consensus sequences deduced for Archaea (without Thermococcaceae) and Bacteria. 173 and 589 refer to the locations of the sites in the 16S rRNA molecules (Escherichia coli numbering). The percentages show the consensus levels. + and −, plus and minus DNA strands, respectively. Mismatches are in lowercase. The site 173 to 193 is highly variable among Bacteria and Eucarya, including variations in size; therefore, the consensus for Thermococcaceae in this region could be compared only with the archaeal consensus. The site 589 to 618 is highly variable among Eucarya, including variations in length, making comparison with Eucarya at this site impossible.