TABLE 1.

Identification of new isolates by PCR with the primer pair TcPc 173F-TcPc 589R, specific for Thermococcaceae

Strain	Isolation source	Cultivation conditionsa				Amplification resultb
		Mineralization of the medium	Electron donor	Electron acceptor	Referencec
SN531	Hydrothermal vent chimney, East Pacific Rise	Marine	Peptone	Fe(III)	12	+
515	Hydrothermal vent chimney, East Pacific Rise	Marine	H2	S0	11	+
AM4	Hydrothermal vent chimney, East Pacific Rise	Marine	CO	None	13	+
AM8	Hydrothermal vent chimney, East Pacific Rise	Marine	Peptone	S0	11	+
SB611	Oil well, North Sea	Marine	Peptone	None	12	+
SM402	Shallow-water marine hydrothermal sediment, Kuril Islands	Marine	Peptone	Fe(III)	12	+
SM4022	Shallow-water marine hydrothermal sediment, Kuril Islands	Marine	Peptone	Fe(III)	12	+
204	Continental hot spring, Kuril Islands	Freshwater	Peptone	S0	4	−
313	Continental hot spring, Kamchatka	Freshwater	Peptone	S0	4	−

^aIn all cases, the media contained 200 mg of yeast extract (Sigma)/liter, the medium pH was 6.5, and the incubation temperature was 85°C.

^b+, single band of the expected size; −, absence of any amplification products.

^cReferences are sources for medium compositions.