TABLE 4.

Proportional change in kinetic parameters for whole cells by quantitative change in isolated cytoarchitectural properties calculated as an effectiveness factor^a

Structural property	Specific affinity	Maximal velocity	Michaelis constant	Substrate threshold
Porin concentration	2	0	−3	−2
Hill constant	−100	0	17	66
Permease concentrationb	2	0	−3	−3
Permease concentrationc	25	7	−3	−3
Site diameter	11	0	−33	−19
Translation coefficient	2	2	−2	−3
Enzyme concentration	31	100	−20	−3
Enzyme affinity	15	0	−10	−2.6

^aAll table values are percent changes in effectiveness factor, ɛ.

^bVariation in permease-dependent transport rates, where rates are further constrained by a limited concentration of cytoplasmic enzymes (Fig. 4C).

^cRates with additional cytoplasmic enzymes (Fig. 4H).