TABLE 1.
Primers used for real-time PCR assays for quantitative detection of respiratory genes
| Target gene | Primera | Sequence | Length of amplicon (bp) | Reference |
|---|---|---|---|---|
| omcB | 8912 | 5′-CCCACTTCGACAACTATTCG-3′ | 212 | This study |
| 8908-2 | 5′-GGTCAGCAGGCCACCGG-3′ | 12 | ||
| omcC | 8917 | 5′-GGTCTTCACCCAGATCTCG-3′ | 232 | This study |
| 8915 | 5′-GGGTGTTGTGGTAGAAGGG-3′ | This study | ||
| frdA | frdA-2 | 5′-GAACTCGGTTACAACGTTG-3′ | 144 | This study |
| frdA-Q5 | 5′-GATGGTGTCATAGAAGAGACG-3′ | This study |
a
Sense and antisense primers for each gene are listed in sequence.